Determination of bile pigment in urine

 Determination of bile pigment in urine

Principle:-


When Barium Chloride reagent is added to urine it combines with sulfate radicals in urine and a precipitate of Barium sulfate is formed if bile pigment is present in the urine then it binds with Fouchet’s  Reagent and green color appearance form.

–Requirement:-

  • Test tube (10×75 mm)
  • Pasture pipette 
  • 10g/dl  barium chloride
  • Fouchet’s reagent 
  • Whatman  no. 1 filter paper (if necessary)
  • Centrifuge 

Procedure:-

  • Take 324 ml of urine in a test tube with the help of a Pasteur pipette. 
  • Add an equal amount of 10g/dl Barium Chloride mix well property.
  • After the mixing of chemical it centrifuges at 1500 RPM for 10 minutes ( filter by using Watermen number 1 filter Paper).
  • After the interview supernatant liquid RD was discarded.
  • At 12 to 2 drop Fouchet’s reagent into the Sediment. ( aur to the precipitate on a filter paper.

Observation: –

Sediment (or to the test tube or on filter papers.

  1. No change in color:- bile pigment absent.
  2. color change to Green:- bile pigment is present.

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