determination of glucose in urine
Name of the test – Benedicts, qualitative Test
principle of the test:-
5ml of a qualitative reagent is heated with an eighth drop (0.5ml) of urine, glucose present in urine reduces the Cupric ion’s presence in the reagent to Cuprous ion. The original color of the Benedict reagent is blue, but it changes to green, yellow orange or red, according to the concentration of glucose present in urine.
Requirment:-
- Test tube
- 5 or 10ml graduated pipette
- 500 ml beaker
- Pasteur pipettes.
- Benedict qualitative reagent
- Bunsen burners.
procedure :-
- -Take a clean and dry test tube.
- Take 5 ml of Benedict’s reagent.
- Add 8 to 10 drops of urine.
- Heat Careful on the Flame of a gas burner. Then place in boiling water (500 ml beaker] for 5 to 10 minutes.
- After 5 to 10 minutes cool under tap water by placing it in a beaker containing tap water and observe the Color of the solution..
- Color is present then sugar is present but the color does not change so sugar is absent.
Observation :-
- Blue———-sugar absent———
- Green and slight yellow———sugar present——250 to 400 mg/dl .
- Green And thick yellow —–sugar present(+ or ++)—-500 to 750 mg/dl
- Yellow or orange—–sugar present (+++)——-1000 to 1500 mg/dl.
- Orange to red——–sugar present (++++) ——more than 1500mg/dl.
