RED BLOOD CELL COUNT

    Stages of RBC count estimation by                       haemocytometer.

    1. Introduction

    2.principle

    3.Requirement

    4.method

    5.procedure

    6.clinical significance

 

    :-Red blood cell count is the important for the diagnosis of haemotological test and it is very important to the calculate the MCV

 

 

 and MCH

   :- the manual method of RBC count is the time consuming .

 

   :- The blood specimen is diluted 200 times with the RBC diluting fluid and cell are counted high power of microscope by the help of neubar counting chamber.

 

   :-microscope

    :-neubauer chamber

    :- RBC diluting fluid

    :- RBC pipette

    :- collection apparatus

       Syringe 💉

      Cotton

      Tourniquate

      Spirit

 

    :- sodium citrate  :3.0 gram

    :- formalin.           : 1.0ml

    :-distilled water    : 100ml

 

   Note  :- this solution is stable at room temperature 🌡️ (25°c) for at least one year

:-Double oxalate or EDTA blood.

:- capillary blood.

 

1.capillary method

 2. Tube method

 

A :- draw the blood up to 0.5 mark in RBC pipette.

 

 B :-carefully wipe the access blood out side the pipette with dry cotton or tissue paper.

 

 C:-draw the RBC diluting fluid up to 101 mark.

 

 D :- mix it properly and wait for 3_5 minute.

 

 E:-discard few drop of diluted blood .

 

 F:-cover slip is placed centrally on neubar counting chamber.

 

 G:- A drop of diluted blood is obtain at top of the pipette and touch the junction of neubar and cover slip and charged the neubar .

 

 H .After charge wait 3 to 5 minute to the settle down of RBC .

 

 I.after waiting ready to the count by Microscope 🥰

 

In male :- 4.5 to 5.5 million cells/ cu mm

In female:- 4.0 to 5.0 million cells / cu M

 

 Method :-2

 All procedure Same only specimen and diluting fluid change .

 Specimen:- 20  microliter or .002 ml

 Diluting fluid :- 3980 microliter or 3.98 ml