determination of serum glucose is very important for human body. Method of determination of serum glucose Glucose oxidase method Principle OF determination of serum glucose :- Normal value glucose (fasting):- Serum glucose (fasting): 70-110 mg/dl, Postprandial (PP): Up to 140…
Introduction of compound microscope:- The compound microscope consists of more than one glass lens in combination. The major lenses are the eye piece, the objective lenses and the condenser lenses. Each of these components is in turn made up of…
BLOOD SUGAR BY FOLIN- WU’S METHOD Principle of Blood sugar by Folin-Bu’s method The protein free filtrate is heated with alkaline cupric tartarate by using special type of Folin-Wu tubes. Glucose (and other reducing substances) present in the filtrate reduce…
Benedict’s Qualitative reagent very important for sugar test. Introduction OF Benedict’s quality reagent Benedict’s reagent (often called Benedict’s qualitative solution or Benedict’s solution) is a chemical reagent and complex mixture of sodium carbonate, sodium citrate, and copper(II) sulfate pentahydrate. It…
Introduction:- Hot air ovens use extremely high temperatures over several hours to destroy microorganisms and bacterial spores. Principle:- When electricity is passed through the heating coils, electrical energy is converted to heat energy. The temperature is controlled by a thermostat. Component:-…
Hot plate and magnetic stirrer PHOTO Introduction of hot plate and magnetic stirrer:- This instrument is used for the fast mixing of solutes in the solution. The magnetic stirrer performs this function. The hot plate provides the necessary heat. Principle…
STANDARDIZATION OF A 1.0 ml VOLUMETRIC PIPETTE Importance:- 1.0 ml volumetric pipette is used for pipetting solutions. For accurate transfer of a solution, the volumetric pipette of 1.0 ml must deliver exactly 1.0 ml of the specimen. Principle:- The volume…
1.Beaker2.Flask3.Measuring cylinder4.Bottle5.Burettes6.Condenser7.Desiccator8.funnels9.Tubes10.Distillation apparatus11.Syringes12.Pipettes Introduction of glassware Glassware used in each section of the laboratory is highly specific and expensive. It is necessary to handle glassware carefully to avoid accidents in the laboratory. Clinical laboratory procedures require accurate determinations to measure…
Introduction:- The spectrophotometer is a routinely used instrument in scientific research spectrophotometer is the quantitative measurement of how much a chemical substance absorbs light by passing a beam of light through a sample using a spectrophotometer. Principle of spectrophotometer The…
Introduction:- A colorimeter is a light-sensitive device that measures the absorbance and transmission of light by a color solution or liquid solution. Principle:- Based on beer’s law And Lambert’s law. Beer’s Law:- According to this law the amount of light…