Ion exchange chromatography ion exchange chromatography In this type of chromatography the stationary phase used is an ion-exchange chromatography resin for separation of constituents based on the number and types of charges present on the column as shown below– Ion-exchanges…
Size exclusion Chromatography (also known as gel filtration Chromatography)
Adsorption Chromatography Adsorption chromatography is a separation technique based on the differential adsorption of components in a mixture onto a stationary phase. Here are some detailed notes on adsorption chromatography: Principle:- Adsorption chromatography relies on the affinity of analytes in…
Chromatography Introduction: – Chromo – color Graphy – writing Tswett was the first to use the chromatography technique. It is a Russian scientist. it describes the separation of color plant pigment he used a column of Aluminium. Yellow – xanthophyll…
Capillary Electrophoresis: – Introduction Principle Component procedure Advantages Introduction: – Capillary electrophoresis is a technique used in analytical chemistry to separate ions based on their electrophoretic mobility in a buffer-filled capillary under the influence of an electric field. It’s widely…
Electrophoresis:- Introduction Principle Requirement Procedure Types 1. Electrophoresis: – The term electrophoresis describes the migration of change particles under the influence of an electric field. 2. Principle: – Many important biological molecules such as amino acids, peptides, proteonucleotides and nucleic…
RADIOIMMUNOASSAY Introduction: – Used to determine the concentrations of antigen present in the blood serum of the patient. Principle:- It is best on competitive binding between the Rio levels antigen (hot antigen) and unlebeled antigen (cold Antigen) with selected antibody…
Serological and Immunological techniques used in the diagnosis of Microbial Infection. Enzyme-Linked Immunosorbent Assay (ELISA):- The term ELISA was introduced by Engvall and Perlmann in 1971 to describe a subset of the widely used immunoassay technique. It is a highly…
Distilled water preparation Introduction:- -Distilled water is a water distillation used to prepare distilled water by using tap water. It is practically free from the interfering ion. principle – water has been boiled by the heating coil changed into vapor…
Flame photometry Introduction:- Flame photometer is an Instrument defined as the measurement of intensity of the light emitted when ony earth metal is introduce in to the flame Based on the element present in the sample it produce emission spectra and…