INTRODUCTION OF SPERM WASHING Importance:- Requirement:- choice of method:- Basic sperm wash method:- 1. Pipette of 2.0 ml liquefied semen aseptically around the bottom centrifuge tube2. Add 2.0 ml sterile sperm washing medium and mix gently3. Centrifuge at 300 g…
microscopic examination of sperm count is very important for the study of morphology of sperm and motility of sperm and also normal range(15 million to 200 million/millimeter) of sperm count is defined by this process. Defined topic name in this…
sperm count Introduction of sperm count Reason for testing Specimen collection(semen/sperm) Requirement Reagent 1. Resorcinal reagent 2. semen diluting fluid 3. Leishman’s stain 1.25 g/dl (Aqueous basic fuchsin All reagent are stable at room temperature physical examination of sperm count…
PHYSICAL Examination of stool Physical examination by visual Observation for the following aspect. 1. Volume 2. Odor 3. Consistency 4. Mucous 5. Blood 6. PH Volume:- 150gm to 260gt Odor:- the normal unpleasant smell associated with stool is a result…
CHEMICAL EXAMINATION OF FECES (STOOL) Occult Blood:- 1. Name of the test 2. Principle 3. Requirement 4. Specimen 5. Procedure 6. Observation Name of the test:- Benzidine test Principle: – Peroxidase type action of hemoglobin in the blood (present in…
Stool examination Stool examination in three ways. 1. Physical examination 2. Chemical examination 3. Microscopic examination Introduction:- The stool is an important specimen for the diagnosis of disease of GIT. Eg:- diarrhea, dysentery, parasitic infection GIT Bleeding … The presence…
Microscopic examination of urine It is studied in the following steps: – 1. Introduction 2. Principle 3. Requirement 4. Instrument 5. Specimen 6. Procedure 7. Observation under a microscope Introduction of Microscopic examination of urine:- The microscopic examination is…
Determination of Urobilinogen Principle: – Urobilinogen reacts with para dimethyl amino benzaldehyde in an acidic medium (in Ehrlich reagent) to form pink colored compound appearance of pink color indicate the presence of urobilinogen in Urine. Method: – Ehrlich’s method Requirement:…
Determination of bile salt Bile:- it is a yellowish color fluid that is secreted by the liver and stored in the gall bladder. Method:- Hay’s Method Principle: – Bile salt when present on the surface tension of urine when sulfur…
Determination of bile pigment in urine Principle:- When Barium Chloride reagent is added to urine it combines with sulfate radicals in urine and a precipitate of Barium sulfate is formed if bile pigment is present in the urine then it…