Category Clinical pathology

Determination of occult blood in urine

occult blood in urine  The chemical method detects free hemoglobin and myoglobin. occult blood in urine Principle of occult blood in urine:- The peroxidase activity of hemoglobin in urine decomposes hydrogen peroxide and oxidized benzidin to form a green-blue color…

Determination of ketone bodies in urine

occult blood in urine

 Ketone body determination in urine  Method :- Rothera test  Principle:-  Nitroprusside used for this test reacts with both acetone and acetoacetic acid in the presence of alkali to produce a purpled Coloured.. –Requirement:- Test tube pipette Test babe back. Liquor…

Determination of Glucose in urine

determination of glucose in urine Name of the test – Benedicts, qualitative Test  principle of the test:-  5ml of a qualitative reagent is heated with an eighth drop (0.5ml) of urine, glucose present in urine reduces the Cupric ion’s presence…

chemical examination of urine

CHEMICAL EXAMINATIONOF URINE chemical examination of urin Includes the Identification of :- 1.protein 2.Glucose 3.Ketone bodies 4.occult blood 5.Bile pigment 6.Bile salts 7.urobilinogen 1.Determination of protein IN URINE Method:- 1.Sulfosalicylic acid test 1.Heat test 1).Sulfosalicylic acid – principles :- If…

Clinical pathology

 Clinical pathology -Clinical pathology is a medical science that is concerned with the diagnosis of disease based on the laboratory analysis of body fluid eg- blood, urine, stool, csf, pericardial fluid, ascitic fluid, synovial Fluid etc. Routine urine examination Urinalysis:-…