Determination of urobilinogen in urine

Principle: –

Urobilinogen reacts with para dimethyl amino benzaldehyde in an acidic medium (in Ehrlich reagent) to form pink colored compound appearance of pink color indicate the presence of urobilinogen in Urine.

Method: – Ehrlich’s method 

Requirement: –

Ehrlich reagent

Barium chloride

Filter paper

Funnel

Test tube 

Rack

Centrifuge 

Pasture pipette

Para dimethyl amino aldehyde – 2ml

Hydrochloric acid -20ml

Distilled water -30ml/day

Procedure:-

Take 5ml urine with the help of pasture pipettes in the clean and dry test tube.

Add 2.5 ml of 10% Barium Chloride.

Mix well.

Filter the solution in another test tube with the help of filter paper what men filter paper.

Filter solution in 0.5 ml of Ehrlich reagent.

Mix well and wait 3 to 5 minutes.

Result:-(Observation) 

No color change  – negative

Development of pink color – urobilinogen present Normal.0

Development of cherry red color – urobilinogen  Increases

Clinical significance: –

Hemolytic Jaundice 

Hepatic Jaundice