how to Sperm Count(semen analysis) in laboratory

sperm count

• sperm count is very important in case of sperm donation and check the fertility.
• normal range of sperm count test is about 15 million to greater than 200 million sperm per milliliter of semen.

• Introduction
• Reason for testing
• Specimen collection
• Requirement
• Reagent
• physical examination of sperm count

Introduction of sperm count

• semen analysis measured the amount of semen a man produced and determined the quality of sperm in the Semen sample.

Reason for testing

• Man has a reproductive problem that is causing in fertility.
• A Vasectomy has been successful.
• it Is also commonly used for testing human Donors for sperm donation.

Specimen collection(semen/sperm)

• 2 to 3 days in an adequate length of abstinence for the proper assessment of semen Quality.
• All patients should be provided with a clean and dry wide mouth glass bottle of plastic container.
• Time of production of semen specimen in the morning for an assessment of motility 6 hour after production.
• Sample should be collected and then reach the lab within 30 minutes after collection because flocculation in temperature may alter the Result.
• The sample container level with patient name identification number date and time of sample collection.

Requirement

• Glass slide
• Coverslip
• Test tube
• Pasture pipette
• Serological pipette
• Neubar Counting Chamber
• Wbc Pipette
• PH paper

Reagent

1. Resorcinal reagent

• Resorcinal:- 50 mg
• conc. HCL :- 33ml
• distilled water :- 67 ml

2. semen diluting fluid

• sodium bicarbonate:- 5 g
• formalin :- 1 ml
• distilled water :- 99 ml

3. Leishman’s stain 1.25 g/dl (Aqueous basic fuchsin

All reagent are stable at room temperature

physical examination of sperm count

• volume
• color
• PH
• Liquefaction of semen

volume of semem

The volume can be measured by using a 1ml graduated Pipette graduated centrifuge tube
Normal volume :- 2-6 mL

color of semen

semen normally has pale grey-yellow opalescent fluid.
Presence of blood:– press the amount of blood the color is a pink and large amount of blood then the color may become brighter.
The presence of bilirubin color of semen is yellow

ph of semen

• The pH of normal fresh semen is between 7.9 to 8.1
• It is measured by using pH paper.

Liquefaction of semen

The human semen is ejaculated in a liquid form and It form jel-like within 5 to 20 minutes.