Pack cell volume :—- it is a parameter of cbc test.
Definition:– when anticoagulant blood ( double oxalate )is centrifused at standard speed 3000 rpm . And heavy part is settled down in bottom . Column of red cell settle is known as pack cell volume or haematocrit volume
METHOD :– there are two methods of determination of pcv
1. Macro-haematocrit volume
2.micro-haematocrit volume
SPECIMEN:–2ml of venous blood
PRINCIPLE:–when EDTA blood centrifused then heavy weight substance that is RBC is packed on the bottom of the tube by the centrifugal force .
REQUIREMENTS:–
– wintrobes tube
– centrifused machine
– EDTA vial
– timer
– cotton swab
– syringe 💉
– tourniquet
TESTING PROCEDURE :–
By wintrobes tube method or macro-haematocrit method
:- collect 2 ml of venous blood in a double oxalate or EDTA vial.
:– mixed well properly.
:- mixed blood is fill in the wintrobes tube or PCV tube by the help of pasture pipette on 100 mark.
:- then centrifused the tube at 300 RPM for 20 to 30 minute.
:- after 30 minute stop the centrifused machine and note the level of pcv by the help of marking .
NORMAL RANGE:—
IN MALE :- 40 to 52 %
In female:- 35 to 48 %
In infant :- 52 to 60 %
