: Haemoglobin study in seven stage
1 introduction
2 method
3 principle
4 Requirements
5 procedure
6 Normal range
7 Clinical significance
-: INTRODUCTION:-
– Haemoglobin is the red respiratory pigment of the RBCs.it composed of haem (iron) and globuline ( protein ) .
– haem ( 4% )
– globuline ( 96% )
– Hemoglobin carried Oxygen (O2) from lungs to the tissue and cells and Carbon dioxide (CO2) from tissue to the lungs .
* When the hemoglobin react with the oxygen to form oxyhemoglobin 97% of oxygen transport in this form and similarly when hemoglobin react with carbon dioxide to form carboxy hemoglobin 30% of CO2 release in this form.
–Hemoglobin act as a good buffer so it maintain acid base balance
– molecular weight of Hemoglobin is 68000 Dalton.
# ESTIMATION OF HEMOGLOBIN
METHOD :-
1 Sahli’s method or acid haematin method
2 Cynameth hemoglobin or Drawkin’s method
3 oxy hemoglobin method
Note:- in all method Cynameth hb% method is more reliable method and acid hematin method is most comman in rural area.
1. SAHLI’S METHOD
PRINCIPLE :- Hemoglobin is converted in to acid hematin with the action of dilute N/10 HCL and after reaction change colour in brown.
2. REQUIREMENTS
1 Comparator box
2.N/10 HCL
3.Hb tube or Sahli’s tube
4.Hb pipette
5.EDTA vial
6. Cottan swab
7. Syringe
8.Diltilled water
9.Dropper
10 . spirit
11. Aplicator
CHEMICAL
Preparation of N/10 HCL :-
8.6 ml concentration of HCL solution is mixed with 100 ml of Distilled water.
SPECIMEN:-
Capillary blood or venous blood 0.02 ml or 20 microlitre.
PROCEDURE:-
1. fill the Sahli’s tube at 2 mark with N/10 HCL
2.then add 0.02 ml blood by the help of pipette .
3. Mixed well and wait 10 minute .
4.after 10 minute change the colour then you are match with coprator box fixed brown strip.
5. If colour of solution and box stip not matched then add distilled water by the help of dropper till matched colour of solution and box brown strip.
6. If colour is matched then stop the adding of d/w and seen the marking of hb tube and give result carefully.
7. When he want results in percentage then you must be multiply by 6.8%
Example
14× 6.8 = 95.2 % hb
Normal range
In adult male 13- 17 g/dl
In adult female 11.6 to 15 g/dl
During pregnancy 9.5 to 12.5 g/dl
Newborn baby 12.5 to 19.5 g/dl
Clinical significance:-
Hb % increase in case
Polycythemia
Vomitting
Hb % decrease in case
Anemia
Chronic disease
Pregnancy
