* ESR *
**Erythrocyte sedimentation Rate
Blood is mixed with anticoagulant and allow to stand on a vertical tube. Seen the rate of Erythrocyte fall-down (setteld download) is known as erythrocyte sedimentation rate and erythrocyte setteled due to the force Called gravitation force towards earth center and erythrocyte is settled down.
principle
– Anticoagulated blood is taken a tube and kept In vertical position in a stand and then allow sedimentation of erythrocyte and force of gravity is used for settle down of erythrocyte then after a spacific time generally 1 hour. the Level of settle down is noted and distance travell by erythrocyte in one hour isliye called erythrocyte sedimentation rate.
-Collection of blood 0.4 mlof 3.8%. Trisodium citrate and 2ml of whole blood is mix well
method
There are three method
1.Western gren method
2.wintrobes method
3. Landu method
Each method of has its advantage and disadvantage by Western gren method more accuracy, result given.It is widely used
Method- Western gren methad
Requirement
Western gren tube
ESR stand
sodium citrate vail
cotton
syringe
Cotton swab
Tourniquate
vial Stand
Testing procedure
1-first of all blood collect in the 3.8% sodium citrated tube.
2.-draw the diluted blood in western gren or ESR tube up to mark zero(0) with the help of pipette and stand in ESR stand .
3.the first note level of settled down in one hour and then 2 hour .
Calculation:-
First hour + second hou/2/2
Normal range
In male:- 0 to 15 mm/hr
In female:- 0 to 20 mside
Note:- western gren tube open both side and wintrobes tube open only one sisir
Thanku