Clinical pathology
-clinical pathology is a medical science that is concerned With the diagnosis of disease based on the laboratory analysis of body fluid, eg- blood, urine, stool, csf, pericardial fluid ,ascitic fluid, synovial Fluid etc.
Routine urine examination.
–urinalysis:- urine analysis can be used to detect Certain diseases such as diabetes, gout, and other metabolic disorder as well as kidney disease.
composition of Normal urine
-Volume 600-2000 ml/day
– Average volume = 1200 ml/day
-spacific gravity = 1.003 to 1.030
-PH= 4.7 to 7.5
-Average PH = 6.0
-Total solid 30 ti70 gram [50% urea]
* following inorganic constituent are excreated Per 24/hour.
-sodium
-Potasium
-calcium
-chloride
-Sulfer
-urea
-Creatine and uricacid
–Harmone and enzyme are detect in small quantity
* Specimen
-collection of urine. in first morning. time some urine avoided then collect in strelize container.
–Preservation:- It should be stored at 2-8°c in refrigerator
-urinalysis should be examine with in one hour of Collection.
*There are three ways to urine examination
1.physical
2.chemical
3.microscopic
1. PHYSICAL EXAMINATION
– physical examination of urine includes description:-
1.color
2.odour
3.clarity
4.Volume
5.spacific gravity
6.reaction and PH.
7.Sediment formation.
Colour: – due to presence of urochrome Pigments.
-The normal urine is straw colour or pale yellow – abnormal urinne colou in Jaundish dark yellowlish.
– in haematuria- redish calour
-abnormal urine colour in bacterial infection- black colour
② odor:- normal urine odor is sweet or fruity smail due to presence of Ketone body.
– Contaminated wine with bacteria. may give pungent smail due to the formation ammonia
– odor of urine in infant – musty odor
③clarity :- normal urine usually clean.
-abnomal urine may appear cloudy, turbid and smoky due to presence of leucocyte,epithelium and Red blood cell
-Volume :-Average volume – 1200 ml/day
-abnormal increase in case of polyuria -> 2500ml/day
-It is generally observed in diabetes.
-abnormal decrease in case of oliguria <5ooml/day.
Specific gravity-Spacific gravity at a constant temp.same as distilled water (for same volume of urine) 1.003 to 1.030.
-High spacific gravity is called Hypersthenuria is fixeed specific gravity = 1.010
-Low spacific gravity is called Hyposthenuria is fixed spacific gravity -1-007-
–PH:– average PH:6.0
Sediment-urine contain solid parts then urine Collect and wait then seen the sediment of haviour component.
Requirement
-pasture pipette
-ordinary filter
-measuring cylinder
-Litmus Paper
-urinometer.
procedures
– Observation of Colour appearance, odor and Sediment by visualization and smailing and note down on report paper.
-Volume It is the measured by help of measuring cylinder
– PH :- is determine by the help of one drop of urine and litmus papers.
–Spacific gravity – determine by the urinometer.
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